A Dual Reporter Splicing Assay Using HaloTag-containing Proteins
نویسندگان
چکیده
منابع مشابه
A Dual Reporter Splicing Assay Using HaloTag-containing Proteins
To evaluate the effects of genetic variations on mRNA splicing, we developed a minigene-based splicing assay using reporter genes encoding luciferase and the multifunctional HaloTag protein. In addition to conventional RT-PCR analysis, splicing events can be monitored in this system using two parameters: luciferase activity and signals derived from HaloTag-containing proteins bound to a fluores...
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ژورنال
عنوان ژورنال: Current Chemical Genomics
سال: 2013
ISSN: 1875-3973
DOI: 10.2174/1875397301206010027